Beneficial interaction of Pomegranate juice and glibenclamide in diabetes induced cardiomyopathy
DOI:
https://doi.org/10.61114/UJPSR.10.1.2026.32-38Keywords:
pomegranate juice, glibenclamide, diabetic cardiomyopathy, cardioprotection, alloxan-induced diabetesAbstract
Diabetes mellitus,a chronic metabolic disorder, is a growing global health crisisthat causes cardiomyopathy through chronic hyperglycemia, oxidative stress, and reactive oxygen species (ROS), damaging heart tissue. Pomegranate juice (Punica granatum), rich in polyphenols and antioxidants, combined with glibenclamide, can target these alloxan-induced complications in rats.This study evaluated the cardioprotective effects of pomegranate juice with glibenclamide against alloxan-induced diabetic complications in rats. Objectives included assessing antinociception via thermal tests, serum biomarkers (LDH, CK-MB), ECG parameters, and antioxidants after 28 days of treatment.Diabetes was induced intraperitoneally with alloxan monohydrate (125 mg/kg); rats exhibiting fasting glucose levels of 210-220 mg/dl were randomised into groups (n=6): normal control, diabetic control, glibenclamide (0.5 mg/kg p.o.), pomegranate juice (3 ml/kg p.o.), and their combination. Cardioprotection was assessed via serum LDH/CK-MB, ECG recordings (heart rate, RR, QT, QRS, ST intervals using physiograph).Diabetic controls showed significant (p<0.001) hyperalgesia, elevated LDH/CK-MB, antioxidant depletion, and ECG changes (increased QRS/PR/QT, decreased RR). Combination therapyreduced biomarkers, restored antioxidants, and corrected ECG, outperforming monotherapies.The combination therapy exhibited potent cardioprotective effects (normalised biomarkers, ECG, antioxidants). Pomegranate juice (3 ml p.o.) + glibenclamide (0.5 mg/kg p.o.) provided superior protection against alloxan-induced diabetic cardiomyopathy compared to monotherapies, suggesting synergistic antioxidant and hypoglycemic mechanisms. These promising preclinical findings warrant further pharmacokinetic, toxicological, and clinical investigations for therapeutic translation.
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Copyright (c) 2026 Shalini Singh, Manodeep Chakraborty, Mohammed Afsal, Jagadish V Kamath
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